Capillary electrophoresis method for screening for affinity ligands using a detectable competitive ligand
US6837977B1 · kind B1 · utility
5Cited by
4References
33Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 23, 2000 |
| Grant date | Jan 4, 2005 |
| Priority date | — |
| Expiry date | Sep 15, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2500/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a capillary electrophoresis-based method of screening complex materials for any unidentified affinity ligand that binds to a target of interest. The method subjects a plug of a mixture of the target and a complex material sample, and a separate plug of a known, tight-binding competitive ligand, to capillary electrophoresis under conditions optimized to allow mingling of the two plugs during the capillary electrophoresis run. Preferably, migration of the competitive ligand is tracked.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.