Method for determining the coagulation potential of a plasma sample
US6838251B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 1, 1999 |
| Grant date | Jan 4, 2005 |
| Priority date | — |
| Expiry date | Feb 1, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/462
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of determining the coagulation potential of a plasma sample be pre-incubating the plasma sample with a reagent such that endogenous protein C in the plasma is at least partially converted into activated protein C by the reagent, adding factor Xa which is progressively inactivated by antithrombin III/heparin cofactor 2 during the preincubation, adding an exogenous reagent which activates factor X to Xa or prothrombin to thrombin in a factor V-dependent manner, monitoring a reaction indicative of the rate of coagulation of the plasma sample, comparing that rate of coagulation control, or the equivalent rate determined for an individual without impaired coagulation control, or the equivalent rate determined for the plasma sample in the absence of protein C activator, and determining the coagulation potential of the plasma sample from one or other of the compairsons.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.