Method for integrating genes at specific sites in mammalian cells via homologous recombination and vectors for accomplishing the same
US6841383B2 · kind B2 · utility
14Cited by
5References
16Claims
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Key dates
| Filing date | Apr 1, 2002 |
| Grant date | Jan 11, 2005 |
| Priority date | — |
| Expiry date | Sep 2, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/44
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Novel vectors and vector combinations for use in the subject cloning method are also provided.
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