Methods for production of arrays with modified oligonucleotide and polynucleotide compositions
US6844151B1 · kind B1 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 29, 1999 |
| Grant date | Jan 18, 2005 |
| Priority date | — |
| Expiry date | Sep 29, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for producing arrays having associated modified nucleic acid structures, e.g. acid stable and/or end-blocked nucleic acids such as 2′-O—R oligonucleotides. In one embodiment, arrays produced using the methods of the invention exhibit an increased binding affinity with complementary nucleic acids, and in particular with complementary RNA. In another embodiment, the associated nucleic acids of the array produced using the methods of the invention exhibit substantial acid resistance, allowing the arrays to be treated with low pH solutions. In another embodiment, the modified associated nucleic acids of the array produced using the methods of the invention exhibit substantial resistance to nuclease degradation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.