Production of tyrosine hydroxylase positive neurons
US6844312B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 9, 2002 |
| Grant date | Jan 18, 2005 |
| Priority date | — |
| Expiry date | Apr 11, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/815
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a method of producing neurons that express the enzyme tyrosine hydroxylase (TH) by subjecting neural stem cells to FGF-1, a protein kinase A activator, a protein kinase C activator, and dopamine/L-DOPA. Surprisingly, when forskolin is used as a protein kinase A activator, it requires only low levels of FGF-1 and forskolin to efficiently produce TH positive neurons from fetal or adult neural stem cells. Also provided are compositions used to produce TH positive neurons and the resulting neural cell culture, as well as a method of treating disease or conditions which are associated with dopamine neuron loss or dysfunction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.