Method for cloning and producing the Msel restriction endonuclease
US6846658B1 · kind B1 · utility
11Cited by
8References
6Claims
0Family size
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Key dates
| Filing date | Oct 12, 2000 |
| Grant date | Jan 25, 2005 |
| Priority date | — |
| Expiry date | Jan 19, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for cloning restriction-modification system is provided whereby the target modification methylase is produced and confers full protection during all growth phases in which the cognate restriction enzyme is present. The method is employed in the cloning of the MseI restriction-modification system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.