Method of producing a recombinant adeno-associated virus, suitable means for producing the same and use thereof for producing a medicament

US6846665B1 · kind B1 · utility

Assignee

• Medi&Gene Inc. · DE

Inventors

• Markus Horer · Martinsried, DE
• Michael Hallek · Cologne, DE

Key dates

• Filing date: Feb 10, 2000
• Grant date: Jan 25, 2005
• Expiry date: Feb 10, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2750/14143
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method for producing a recombinant adeno-associated virus (rAAV), in which at different times a helper construct and also a vector construct are introduced into a suitable cell, and preferably the helper construct does not comprise, in particular with the exception of the AAV promoters, any nucleic acid sequences to which at least a Rep protein can essentially specifically bind and preferably the vector construct comprises ITR sequences in flop orientation. The recombinant adeno-associated viruses produced according to the method of the invention are suitable in particular for producing a tumor cell into which additionally nucleic acids coding for GM-CSF and B7.2 has been introduced, which in turn can be used in the form of a medicament for the treatment of cancers.