PCR-based monitoring in wastewater biotreatment systems
US6849430B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 23, 2002 |
| Grant date | Feb 1, 2005 |
| Priority date | — |
| Expiry date | Jul 29, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/158
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
PCR-based monitoring of microorganisms in systems for the biological treatment of wastewater. In particular, measuring both the abundance and expression of indicator/effector genes or gene combinations, where expression of an “effector” gene correlates with the degradative activity of a particular microbial sample, and where the abundance of an “indicator” gene correlates with the abundance of microbe. Effector gene expression is measured by competitive quantitative RT-PCR, and indicator gene abundance is measured by competitive quantitative PCR. The indicator and effector genes may be the same or different genes. In one embodiment, the indicator and effector gene are sequences from the glyphosate oxidoreductase (gox) gene and the quantitative techniques employed are competitive quantitative PCR and competitive quantitative RT-PCR.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.