Method for producing high methionine corn seeds
US6849779B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 25, 1999 |
| Grant date | Feb 1, 2005 |
| Priority date | — |
| Expiry date | Aug 25, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8253
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides novel DNA constructs encoding high methionine zein proteins, the expression of which is not negatively regulated by the dzr1 regulatory protein. The constructs of the invention comprise a δ-zein coding region operably linked to a promoter and a 3′ UTR which has been modified so as to be devoid of any binding sites for the dzr1 regulatory protein. Preferably, the entire 3′ UTR is replaced by a heterologous sequence that does not contain any dzr1 binding sites. Transgenic corn plants comprising the DNA constructs of the invention are also provided. These plants consistently produce high methionine corn seeds.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.