Compositions and methods for utilizing mixed substrate solutions of luminols and dioxetanes
US6852503B1 · kind B1 · utility
6Cited by
5References
5Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 29, 2002 |
| Grant date | Feb 8, 2005 |
| Priority date | — |
| Expiry date | Jul 22, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/28
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A dual enzyme chemiluminescent substrate formulation useful for the independent or simultaneous analysis of the enzymes is disclosed, the formulation includes, as one substrate component, dihydrophthalazinedione, such as luminol or isoluminol, and a peroxide source and, as the other substrate component, a 1,2-dioxetane. The formulation further includes a polymeric quaternary onium salt as an isolating agent to defeat adverse interactions between the substrate pairs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.