DNA isolation method
US6852851B1 · kind B1 · utility
65Cited by
3References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 20, 2000 |
| Grant date | Feb 8, 2005 |
| Priority date | — |
| Expiry date | Oct 20, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membrane and at least some of the nuclear membranes, and introducing the lysate into micro-channels of a microfabricated apparatus in which each of the micro-channels is provided with a barrier disposed in the channel to impede the passage or flow of DNA and cell nuclei each while allowing the passage of liquid through the micro-channel so that a mesh comprising DNA is formed in the channel.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.