Method for increasing viability and transformation efficiency of bacteria during storage at low temperatures
US6855494B2 · kind B2 · utility
2Cited by
25References
31Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 8, 1999 |
| Grant date | Feb 15, 2005 |
| Priority date | — |
| Expiry date | Jan 8, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to improved E. coli bacteria with enhanced viability at low temperatures, methods for producing improved bacterial strains capable of enhanced viability at low temperatures, and the isolation and use of genetic material capable of enhancing the viability of bacteria at low temperatures. In addition to the enhanced viability at low temperatures, the bacteria may exhibit enhanced transformation efficiencies after storage at low temperatures. As such, the invention may be used for the insertion of exogenous DNA sequences into the bacteria of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.