Heterocyclic FRETdye cassettes for labeling biological molecules and their use in DNA sequencing
US6855503B2 · kind B2 · utility
3Cited by
3References
19Claims
0Family size
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Key dates
| Filing date | Dec 20, 2002 |
| Grant date | Feb 15, 2005 |
| Priority date | — |
| Expiry date | Dec 20, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBasic materials chemistry
- WIPO sectorChemistry
Abstract
Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.