Dilutions in high throughput systems with a single vacuum source
US6858185B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 23, 2000 |
| Grant date | Feb 22, 2005 |
| Priority date | — |
| Expiry date | Oct 23, 2020 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/2575
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Flow rates in a microfluidic device are modulated after performing serial dilutions by flow reduction channels that draw fluid from the main channel, thus reducing the flow rate. The reduction in flow rate and/or use of smaller dimension channels allow reduced reagent consumption. In addition, multiple flow reduction channels are used for multiple concentration measurements and for performing multiple assays simultaneously on a single sample. Also included are microfluidic devices and integrated systems for performing assays using serial dilutions, single pressure sources, multiple concentration measurements, and reduced reagent consumption. Devices comprising flow reduction channels are also used to suppress pressure perturbations from spontaneous injection.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.