Methods for stable transformation of plants

US6858777B2 · kind B2 · utility

Assignee

• Syngenta Participations AG · CH

Inventors

• Heng Zhong · Темневкий пруд, US
• Eric Boudreau · Saint-Lambert-de-Lauzon, CA
• Sabrina Rouse · Clayton, US
• Erik Dunder · Chapel Hill, US
• Weining Gu · Morrisville, US
• Yin-Fu Chang · Carrboro, US

Key dates

• Filing date: Aug 13, 2001
• Grant date: Feb 22, 2005
• Expiry date: Sep 10, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8205
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash, Brassica, and pepper.