Method and apparatus for rapid change of fluorescence bands in the detection of dyes in fluorescence microscopy
US6858852B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 7, 2001 |
| Grant date | Feb 22, 2005 |
| Priority date | — |
| Expiry date | Apr 27, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6421
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is disclosed for operation of an image-generating optical system for detection of characteristic quantities of the wavelength-dependent behavior of an illuminated specimen, such as the emission behavior and/or absorption behavior and, in particular, the fluorescence and/or luminescence and/or phosphorescence and/or enzyme-active light emission and/or enzyme-active fluorescence, for the operation of a laser scanning microscope, comprising the steps of splitting the image point information of the specimen into spectral components in a spatially resolved manner on the detection side in dependence on wavelength and carrying out at least one summing for different spectral components.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.