Genes containing a DNA sequence coding for hydroxynitrile lyase, recombinant proteins derived therefrom and having hydroxynitrile lyase activity, and use thereof

US6861243B2 · kind B2 · utility

Assignee

• DSM Fine Chemicals Austria NFG GmbH & Co KG · AT

Inventors

• Helmut Schwab · Graz, AT
• Anton Glieder · Gleisdorf, AT
• Christoph Kratky · Graz, AT
• Ingrid Dreveny · Graz, AT
• Peter Pochlauer · Dordrecht, NL
• Wolfgang Skranc · Wien, AT
• Herbert Mayrhofer · Linz, AT
• Irma Wirth · Ansfelden, AT
• Rudolf Neuhofer · Mittertreffling, AT
• Rodolfo Bona · Graz, AT

Key dates

• Filing date: Jan 16, 2002
• Grant date: Mar 1, 2005
• Expiry date: Apr 2, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of the 5′-region of the mdl genes from Prunus serotina and from Prunus amygdalus and/or a primer 2 based on the 3′-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes from Prunus serotina or from Prunus amygdalus, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)- or (S)-cyanohydrins.