Production of heterologous polypeptides from freshwater caulobacter
US6861245B1 · kind B1 · utility
7Cited by
4References
20Claims
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Key dates
| Filing date | Feb 21, 2000 |
| Grant date | Mar 1, 2005 |
| Priority date | — |
| Expiry date | Feb 21, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for screening Caulobacter suitable for use as host organisms for secretion of heterologous polypeptides. Such Caulobacter have a transport protein homologous to one of the type I transport proteins known in C. crescentus. DNA constructs are also provided which code for a chimeric protein of which the C-terminus is a secretion signal of a Caulobacter surface layer protein, other than from C. crescentus. Bacterial cells containing, or which express such DNA constructs and which may secrete the resulting protein, are also provided.
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