Sheathless interface for capillary electrophoresis/electrospray ionization-mass spectrometry using an in-capillary electrode
US6863790B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 5, 1999 |
| Grant date | Mar 8, 2005 |
| Priority date | — |
| Expiry date | Aug 5, 2019 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44717
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A simple and rugged sheathless interface for capillary electrophoresis/electrospray ionization-mass spectrometry (CE/ESI-MS) was designed using common laboratory tools and chemicals. The interface uses a small platinum (Pt) wire which is inserted into the CE capillary through a small hole near the terminus. The position of the wire inside the CE capillary and within the buffer solution is analogous to standard CE separation operations where the terminus of the CE capillary is placed inside a buffer reservoir along with a grounded platinum electrode. By combining the use of the in-capillary electrode interface with sharpening of the fused silica tip of the CE capillary outlet, a stable electrospray current was maintained for an extended period of time. The design was successfully applied to CE/ESI-MS separations and analysis of mixtures of peptides and proteins. A detection limit of approximately 4 femtomole (S/N=3) was achieved for detection of myoglobin utilizing a 75 μm-i.d. aminopropylsilane treated CE column and using a wide scan range of 550-1350 Da. The advantages of this new design include: (1) a stable CE and ESI current, (2) durability, (3) a reduced risk of sparking betwe…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.