Method for producing hybridization complexes whose stability is substantially independent of the base composition of two hybridized nucleic acid molecules
US6864049B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 25, 1997 |
| Grant date | Mar 8, 2005 |
| Priority date | — |
| Expiry date | Jun 25, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6832
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention discloses a method for producing a hybridization complex whose stability is substantially independent of the base composition of two hybridized nucleic acid molecules, consisting in contacting a first nucleic acid molecule with a second nucleic acid molecule under conditions enabling the formation of a specific hybridization complex. The invention is characterized in that at least one of the four types of bases contained in the sequence of the first and/or of the second nucleic acid molecule is a modified base having pairing properties close to those of the natural base it replaces, such that at least one of the two types of the base pair contained in the composition of the complex comprises a modified base and in that the totality of the base pairs contained in the composition of the said complex has a like stability.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.