Vaccinia topoisomerases I-based assays for detection of specific DNA breaks
US6864058B2 · kind B2 · utility
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50Claims
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Key dates
| Filing date | Feb 6, 2002 |
| Grant date | Mar 8, 2005 |
| Priority date | — |
| Expiry date | Feb 6, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Several types of DNA cuts are used as markers of apoptosis for detection of apoptotic cells in situ. The present invention includes a method to detect DNase II type DNA damage, bearing 5′OH using vaccinia topoisomerase I. The present invention also includes a method combining a ligase-based method to detect DNase I type DNA damage and the topoisomerase based method, resulting in simultaneous detection of two specific types of DNA damage in situ.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.