Affinity selected signature peptides for protein identification and quantification
US6864099B2 · kind B2 · utility
13Cited by
4References
32Claims
0Family size
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Inventor
Key dates
| Filing date | May 4, 2001 |
| Grant date | Mar 8, 2005 |
| Priority date | — |
| Expiry date | May 4, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for protein identification in complex mixtures that utilizes affinity selection of constituent proteolytic peptide fragments unique to a protein analyte. These “signature peptides” function as analytical surrogates. Mass spectrometric analysis of the proteolyzed mixture permits identification of a protein in a complex sample without purifying the protein or obtaining its composite peptide signature.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.