Automated protein purification the multiwell format by vacuum filtration

US6864100B1 · kind B1 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Joachim Ribbe · Düsseldorf, DE
• Frank Schäfer · Weilheim, DE
• Kerstin Steinert · Langenfeld, DE
• Helge Lubenow · Solingen, DE

Key dates

• Filing date: Aug 2, 2000
• Grant date: Mar 8, 2005
• Expiry date: Aug 2, 2020

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The invention relates to a process and a reagent kit for obtaining clear solutions containing cell contents from biological samples in a high throughput process, comprising the steps of preparing a plurality of protein-containing solutions which contain insoluble ingredients, in separate chambers of a multi-chamber filtration unit, eliminating insoluble ingredients by filtering the solutions through the multi-chamber filtration unit with the application of a pressure differential, while preventing cross contamination between adjacent chambers by chemical and/or mechanical means, and collecting the individual filtrates separately in collecting containers, as well as a process for recovering cell contents from the clear solutions obtained. The invention further relates to the use of an apparatus for recovering clear protein-containing solutions from biological samples.