Stepped electrophoresis for movement and concentration of DNA
US6866759B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 13, 2000 |
| Grant date | Mar 15, 2005 |
| Priority date | — |
| Expiry date | Jan 14, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44773
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluidic channel patterned with a series of thin-film electrodes makes it possible to move and concentrate DNA in a fluid passing through the fluidic channel. The DNA has an inherent negative charge and by applying a voltage between adjacent electrodes the DNA is caused to move. By using a series of electrodes, when one electrode voltage or charge is made negative with respect to adjacent electrodes, the DNA is repelled away from this electrode and attached to a positive charged electrode of the series. By sequentially making the next electrode of the series negative, the DNA can be moved to and concentrated over the remaining positive electrodes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.