Cellular assays for identifying signaling peptides
US6866998B1 · kind B1 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 26, 1998 |
| Grant date | Mar 15, 2005 |
| Priority date | — |
| Expiry date | Nov 26, 2018 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2799/027
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention includes a DNA encoding a known translocatable protein, for example, human mpl, lacking secretory ability due to deletion of its secretory signal and the major part of the extracellular region thereof. This DNA is present in an expression vector into which a test cDNA from a cDNA library is ligated. The expression vector containing the known DNA and the test cDNA expresses a chimeric protein encoded by these two nucleic acid molecules. Cells expressing the newly constructed vector are capable of proliferating if the test cDNA is a secretory protein. Thus, cDNAs encoding secretory proteins including type I membrane proteins and type II membrane proteins can be detected and isolated by constructing a cDNA library and screening it for ability to promote cell proliferation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.