Method for producing chiral carboxylic acids from nitriles with the assistance of a nitrilase or microoganisms which contain a gene for the nitrilase

US6869783B1 · kind B1 · utility

Assignee

• BASF SE

Inventors

• Marion Ress-Löschke · Dossenheim, DE
• Thomas Friedrich · Darmstadt, DE
• Bernhard Hauer · Neheim, DE
• Ralf Mattes · Stuttgart, DE
• Dirk Engels · Neckartenzlingen, DE

Key dates

• Filing date: Oct 13, 1999
• Grant date: Mar 22, 2005
• Expiry date: Oct 13, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P41/006
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to nucleic acid sequences which code for a polypeptide having nitrilase activity, to nucleic acid constructs comprising the nucleic acid sequences, and to vectors comprising the nucleic acid sequences or the nucleic acid constructs. The invention further relates to amino acid sequences which are encoded by the nucleic acid sequences, and to microorganisms comprising the nucleic acid sequences, the nucleic acid constructs or vectors comprising the nucleic acid sequences or the nucleic acid constructs.The invention additionally relates to a process for preparing chiral carboxylic acids from the racemic nitriles.