Multi-color multiplexed analysis in a bio-separation system
US6870165B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 21, 2002 |
| Grant date | Mar 22, 2005 |
| Priority date | — |
| Expiry date | Oct 21, 2022 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A multi-channel and multi-color bio-separation detection method and apparatus in which a single detector is coupled to a plurality of radiation sources, in a one detector/many radiation sources configuration. Each radiation source directs radiation at a detection zone of a single separation channel, and a single detector is applied to detect light emissions from the detection zones associated with several radiation sources. The radiation sources are activated to direct radiation at the detection zone in a predetermined sequence and further in a cyclic manner, with the detector output synchronized to the radiation sources by a controller. Bio-separation may be conducted simultaneously in all the channels in parallel, with detection time-staggered and/or time multiplexed with respect to the light sources. In one embodiment, low cost light emitting diodes may be used as radiation sources. In another aspect, the detection scheme is configured for radiation induced fluorescence detection in an capillary electrophoresis instrument.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.