Complexity management of genomic DNA
US6872529B2 · kind B2 · utility
35Cited by
43References
43Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Dec 10, 2001 |
| Grant date | Mar 29, 2005 |
| Priority date | — |
| Expiry date | Apr 8, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The presently claimed invention provides for novel methods and kits for reducing the complexity of a nucleic acid sample by providing non-gel based methods for size fractionation. In a preferred embodiment, size fractionation can be accomplished by varying conditions or reagents of a PCR reaction to amplify fragments of specific size ranges. The invention further provides for analysis of the above sample by hybridization to an array, which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.