Method of reconstituting nucleic acid molecules
US6872552B2 · kind B2 · utility
Inventor
Key dates
| Filing date | Feb 27, 2001 |
| Grant date | Mar 29, 2005 |
| Priority date | — |
| Expiry date | Apr 24, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/68
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a system for reconstituting nucleic acid molecules that have been degraded but still contain useful genetic information. The present invention uses, as a template for reconstituting degraded nucleic acids in a biological sample, nucleic acids from a genetically related or identical organism having a sequence homologous to the degraded nucleic acids. After hybridization of the degraded nucleic acids to the template, regions of the degraded nucleic acids that are missing in the duplex containing template nucleic acid molecules hybridized to degraded nucleic acids are filled in with nucleotides using the intact nucleic acid molecule as a template. The newly formed strand of nucleic acid is used as the template for a subsequent step of hybridization to degraded nucleic acid molecules. Regions of degraded nucleic acids that are missing in the duplex are again filled in with nucleotides using the newly formed nucleic acid as the new template. The process is repeated until the newly formed nucleic acid molecules are substantially representative of the nucleic acids from the genome of the species from which the degraded sample is obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.