Compositions and methods for production of disulfide bond containing proteins in host cells

US6872563B1 · kind B1 · utility

Assignees

• President and Fellows of Harvard College · US
• The Board of Regents of the University of Texas System · US

Inventors

• Jonathan Beckwith · Cambridge, US
• Fredrik Aslund · Stockholm, SE
• Paul H. Bessette · Camarillo, US
• George Georgiou · Austin, US
• Daniel Ritz · Allschwil, CH
• Jackie Eun-ah Lim · Shrewsbury, US

Key dates

• Filing date: Oct 5, 2000
• Grant date: Mar 29, 2005
• Expiry date: Feb 17, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides composition and methods for producing proteins of interest which comprise at least one disulfide bond, include proteins which in their mature form do not contain disulfide bonds, but whose precursor molecule contained at least one disulfide bond. The methods employ a host cell modified to more efficiently produce properly folded disulfide bond containing proteins. The host cells generally contain a mutation in one or more reductase genes, and can be further genetically modified to increase their growth rate, and are further optionally modified to increase the expression of a catalyst of disulfide bond formation. Host cells, methods for using such to produce proteins of interest, proteins of interest produced by these methods are within the scope of the invention.