Ammonium sulfate for neutralization of inhibitory effects

US6872818B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Christian Korfhage · Hilden, DE
• Ralf Wyrich · Grevenbroich, DE
• Uwe Oelmüller · Erkrath, DE

Key dates

• Filing date: Jan 25, 2002
• Grant date: Mar 29, 2005
• Expiry date: Jan 25, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/1003
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides RNA purification and/or analysis methods that use ammonium sulfate to mitigate or neutralize inhibitory effects of certain molecules. Exemplary inhibitory molecules that interfere with RNA function or analysis are those that bind to or cleave RNA, or stabilize RNA secondary structures.