Method for isolating and recovering target DNA or RNA molecules having a desired nucleotide sequence
US6875568B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 10, 2001 |
| Grant date | Apr 5, 2005 |
| Priority date | — |
| Expiry date | Apr 10, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6813
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention generally concerns the use of amino acid denaturants for denaturing or separating double stranded nucleic acid molecules. More specifically, the present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of haptenylated probes and amino acid denaturants to select the desired molecules and eliminate the undesired library members from a sample. The invention also provides a method in which larger or full-length nucleic acid molecules can be isolated from the subpopulation of desired molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.