Ultrasensitive immunoassays
US6878515B1 · kind B1 · utility
144Cited by
26References
8Claims
0Family size
Inventor
Key dates
| Filing date | Jun 14, 1996 |
| Grant date | Apr 12, 2005 |
| Priority date | — |
| Expiry date | Jan 12, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to an immunological test kit and immunoassay using a first immobilized antibody having affinity for a specific antigen. The invention is characterized by a second and third antibody being specific for different determinants of the antigen and modified with cross-linkable oligonucleotides. For detection, the oligonucleotides are amplified, whereby only such oligonucleotides will be amplified which have been cross-linked to each other. In this way unspecific background is avoided and detection is possible down to single molecules.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.