Transposable element-anchored, amplification method for isolation and identification of tagged genes
US6881539B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 16, 1999 |
| Grant date | Apr 19, 2005 |
| Priority date | — |
| Expiry date | Feb 16, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1082
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the rapid isolation and identification of the DNA sequence of transposable element-tagged genes is provided. The method comprises a modified AFLP approach using a transposable element-anchored amplification to identify and clone an amplification product that is associated with a mutant phenotype. Once cloned, the amplification product of interest may be used to screen a cDNA library directly or may be sequenced and compared to available databases for sequence homology. A modification of this approach provides a method for the identification of the location of an additional type of insertion event into genomic DNA, more specifically transgene insertion into the genome of a host organism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.