Method for analyzing a fluid sample

US6881541B2 · kind B2 · utility

Assignee

• Cepheid · US

Inventors

• Kurt E. Petersen · San Jose, US
• Michael T. Taylor · Newark, US
• Farzad Pourahmadi · Fremont, US
• William A. McMillan · Cupertino, US
• Ronald Chang · Redwood City, US
• Stanley H. Sakai · Cupertino, US
• Jesus Ching · Saratoga, US
• Douglas B. Dority · Santa Cruz, US
• Phillip Belgrader · Severna Park, US
• M. Allen Northrup · Berkeley, US

Key dates

• Filing date: Mar 6, 2001
• Grant date: Apr 19, 2005
• Expiry date: Dec 12, 2021

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for extracting nucleic acid from a fluid sample comprises the steps of introducing the sample into a cartridge having a sample flow path and a lysing chamber in the sample flow path. The lysing chamber contains at least one filter for separating cells or viruses from the sample. The sample is forced to flow through the lysing chamber to capture the cells or viruses with the filter, while used sample fluid flows to waste. The captured cells or viruses are disrupted to release their nucleic acid, the nucleic acid is eluted from the lysing chamber, and optionally the nucleic acid is amplified and detected in a reaction chamber of the cartridge.