Mutant B-type DNA polymerases exhibiting improved performance in PCR

US6881559B2 · kind B2 · utility

Assignee

• Roche Diagnostics GMBH · DE

Inventors

• Harald Sobek · Penzberg, DE
• Bruno Frey · Penzberg, DE
• Garabed Antranikian · Seevetal, DE
• Kristina Boehike · Berlin, DE
• Francesca Maria Pisani · Roma, IT
• Mose Rossi · Napoli, IT

Key dates

• Filing date: Mar 9, 2001
• Grant date: Apr 19, 2005
• Expiry date: Mar 9, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1252
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to thermostable mutants of B-type DNA polymerases comprising a Y-GG/A amino acid motif between the N-terminal 3′-5′-exonuclease domain and the C-terminal polymerase domain whereas the tyrosine of the Y-GG/A amino acid motif is mutated and whereas these mutant DNA polymerases are suitable for PCR.