Methods for screening compounds for estrogenic activity

US6884577B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Peter J. Kushner · San Francisco, US
• Paul Webb · Leeds, GB
• Renee Williard · San Francisco, US
• C. Anthony Hunt · San Francisco, US
• Gabriella Lopez · San Francisco, US

Key dates

• Filing date: Dec 4, 2000
• Grant date: Apr 26, 2005
• Expiry date: Jul 17, 2021

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides novel assay methods for identifying compounds that may have both estrogen agonist and antagonist properties. In particular, the assay use cells comprising promoters having an AP1 site linked to a reporter gene. Compounds capable of inducing or blocking expression of the reporter gene can thus be identified. The compounds may be further tested for the ability to modulate the standard estrogen response, as well.