Patent · US Expired

Expression of alkaline phosphatase in yeast

US6884602B2 · kind B2 · utility

10Cited by

0References

7Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Rainer Mueller · Penzberg, DE
Johann-Peter Thalhofer · Weilheim, DE
Frank Geipel · Penzberg, DE
Werner Hoelke · Penzberg, DE
Stephan Glaser · Seeshaupt, DE
Hellmut Eckstein · Weilheim, DE
Thomas Kirschbaum · Munich, DE
Bettina Bommarius · Atlanta, US

Key dates

Filing date	Jul 23, 2001
Grant date	Apr 26, 2005
Priority date	—
Expiry date	Jul 23, 2021

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y301/03001
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

This invention involves a process for selecting and producing eukaryotic alkaline phosphatase in yeast. Yeast cells are subjected to multiple transformations using a vector comprising a first resistance marker gene and the alkaline phosphatase gene. Those strains that grow in media containing the first resistance marker are further transformed using a vector comprising a second selection marker gene and the alkaline phosphatase gene. Transformants that grow in media containing the second selection marker are selected for expressing the eukaryotic alkaline phosphatase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.