Modified rapid expansion methods(“modified-REM”) for in vitro propagation of T lymphocytes

US6890753B2 · kind B2 · utility

Inventors

• David Flyer · Olney, US
• Kim W. Clary · Seattle, US

Key dates

• Filing date: Sep 17, 2001
• Grant date: May 10, 2005
• Expiry date: Sep 17, 2021

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2502/11
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides a modified rapid expansion method (termed “low-PBMC-REM” or “modified-REM”), for quickly generating large numbers of T lymphocytes, including cytolytic and helper T lymphocytes, without using the large excesses of peripheral blood mononuclear cells (PBMC) or EBV-transformed lymphoblastoid cells (LCL) characteristic of high-PBMC-REM. Clonal expansions of greater than 500-fold can be achieved within a single stimulation cycle of about 8-14 days.