Methods for improving conifer embryogenesis
US6893873B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 27, 2003 |
| Grant date | May 17, 2005 |
| Priority date | — |
| Expiry date | Feb 2, 2023 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H4/002
- WIPO fieldFood chemistry
- WIPO sectorChemistry
Abstract
The present invention provides methods for initiating, capturing, maintaining and multiplying embryogenic cultures of coniferous plants. Methods include the use of novel media compositions containing Vitamin B12, Vitamin E, or organic acids including α-ketoglutaric acid, pyruvic acid, or p-aminobenzoic acid to improve the frequency of embryogenic tissue initiation, capture, maintenance and multiplication. The methods are well suited for initiating embryogenic cultures in recalcitrant conifer varieties. The method is also well suited for producing somatic embryos that can be further cultured to produce large numbers of plants. Further, the invention provides novel methods that may be used to enhance somatic embryogenesis in a broad range of species.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.