Miniaturized temperature-zone flow reactor

US6896855B1 · kind B1 · utility

Assignee

• Institut fuer Physikalische Hochtechnologie E.V. · DE

Inventors

• Johann Michael Köhler · Golmsdorf, DE
• Andreas Mokansky · Dresden, DE
• Siegfried Poser · Jena, DE
• Torsten Schulz · Pinneberg, DE

Key dates

• Filing date: Feb 10, 1999
• Grant date: May 24, 2005
• Expiry date: Feb 10, 2019

Classification

• Technology area (CPC B): Performing Operations; Transporting
• CPC primary: B01L2300/1883
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

A miniaturized temperature-zone flow reactor, used for thermally controlled biochemical or molecular-biological processes, especially polymerase chain reaction (PCR) enables more efficient reactions by providing at least one closed flow path which is divided into three partial paths (A1 . . . An; B1 . . . Bn and BB1 . . . BBn−1; C1 . . . Cn) with the reactor having three substrate chips (A; B; C) which are made of a material having a high heat conductivity, and which have defined channel sections that are spaced apart relative to each other, and are connected by a connecting chip (V) made of a poor heat-conductive material. The substrate chips (A; B; C) are maintained at different temperatures by various means, including the use of controlling heating elements in contact with the chips.