Multiparameter FACS assays to detect alterations in exocytosis
US6897031B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 17, 1998 |
| Grant date | May 24, 2005 |
| Priority date | — |
| Expiry date | Apr 17, 2018 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/4718
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Described is a method for screening for alterations in exocytosis of a population of cells. The cells are sorted by a FACS machine by assaying for alterations in at least three of the properties selected from the group consisting of light scattering, fluorescent dye uptake, fluorescent dye release, annexin granule binding, surface granule enzyme activity, and the quantity of granule specific proteins. Methods for screening for bioactive agents capable of modulating exocytosis in a cell are also described. The methods provide for reduced background and increased specificity without increasing the time or steps involved in assaying for exocytosis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.