Process for labeling a nucleic acid
US6902891B2 · kind B2 · utility
6Cited by
33References
49Claims
0Family size
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Key dates
| Filing date | Dec 15, 2000 |
| Grant date | Jun 7, 2005 |
| Priority date | — |
| Expiry date | Oct 23, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2523/107
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A process of fragmenting and labeling a synthetic or natural nucleic acid, comprising the steps of providing a mixture containing a nucleic acid, a labeling agent containing a detectable label, and at least one multivalent metal cation in a substantially aqueous solution; chemically fragmenting the nucleic acid in the mixture to produce a multiplicity of nucleic acid fragments; and attaching at least one label to at least one of the nucleic acid fragments to produce a detectably labeled nucleic acid fragment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.