Recombinant DNA processes using a dNTP mixture containing modified nucleotides

US6902914B2 · kind B2 · utility

Assignee

• Sigma-Aldrich Co. LLC · US

Inventors

• Brian Ward · Chesterfield, US
• Leanne Snyder · Chesterfield, US
• Chuan Li · Chesterfield, US
• Keming Song · Ballwin, US
• Kristen Opper · Collinsville, US
• Stephanie Uder · Edwardsville, US
• Ron Hernan · Ballwin, US

Key dates

• Filing date: Nov 15, 2001
• Grant date: Jun 7, 2005
• Expiry date: Apr 23, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6846
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Amplification mixtures, kits, amplicons, kits and processes are provided for amplifying a nucleic acid. In particular, provided are processes which utilize an amplification mixture comprising a polymerase, a deoxynucleotidetriphosphate (dNTP) mixture which contains modified dNTPs, a first primer and a second primer. Further provided are dNTP mixtures which contain modified dNTPs for at least two of the four nucleotide triphosphates, which when incorporated into a polynucelotide, impart resistance to enzymatic degradation by an exonuclease at the sites of incorporation of the modified dNTPs. Also provided are the amplicons and vectors which incorporate the modified nucleotides.