Method for producing an active heterodimeric AMV-RT in prokaryotic cells

US6902920B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Harald Sobek · Penzberg, DE
• Rainer Mueller · Penzberg, DE
• Manfred Schmidt · Krefeld, DE
• Bruno Frey · Penzberg, DE
• Bernhard Suppmann · Weilheim, DE
• Rainer Schmuck · Benediktbeuern, DE
• Johann-Peter Thalhofer · Weilheim, DE
• Peter Pallua · Wien, AT
• Markus Pajatsch · Munich, DE

Key dates

• Filing date: Sep 21, 2001
• Grant date: Jun 7, 2005
• Expiry date: May 9, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/1276
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The heterologous expression of the reverse transcriptase from the Avian Myeloblastosis Virus (AMV-RT) in prokaryotic cells and in particular Escherichia coli (E. coli) is described in the present invention. The invention also includes certain measures to simplify the purification of the heterodimeric AMV-RT.