Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells
US6903193B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 10, 2000 |
| Grant date | Jun 7, 2005 |
| Priority date | — |
| Expiry date | Nov 10, 2020 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y402/03006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells, isolated trichodiene synthases and nucleic acid sequences encoding the trichodiene synthases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.