Conjugated ligands for the stimulation of blood cell proliferation by effecting dimerization of the receptor for stem cell factor

US6904369B1 · kind B1 · utility

Assignees

• The Trustees of Columbia University in the City of New York · US
• AMGEN INC. · US

Inventors

• Wayne A. Hendrickson · Dobbs Ferry, US
• Xuliang Jiang · Braintree, US
• Keith E. Langley · Newbury Park, US
• Rashid Syed · Thousand Oaks, US
• Yueh-Rong Ann Hsu · Los Angeles, US

Key dates

• Filing date: Jun 29, 2000
• Grant date: Jun 7, 2005
• Expiry date: Sep 25, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/16022
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides a computer based method for preparing a stem cell factor (SCF) analog comprising the steps of: (a) providing computer expression of the three dimensional structure of an SCF molecule using its crystal structure; (b) selecting from the computer expression of step (a) at least one site on the SCF molecule for alteration; (c) preparing a SCF molecule having an alteration at said at least one selected site; and (d) optionally, testing the SCF molecule for a desired characteristic. This invention also provides SCF analogs and SCF ligand analogs prepared according to the above-described method. Compositions comprising SCF analogs or SCF ligand analogs prepared according to the above-described method effective to treat a subject and a pharmaceutically acceptable carrier are provided, as are methods of treating a subject comprising administration of pharmaceutical compositions comprising the prepared SCF analogs and SCF ligand analogs prepared by the described methods. This invention also provides methods for designing compounds capable of binding to the SCF receptor site and compounds designed by the above-described methods.