Method for the identification and characterization of interacting molecules by automated interaction mating

US6905818B1 · kind B1 · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Erich Wanker · Berlin, DE
• David BANCROFT · Gilching, DE
• Hans Lehrach · Berlin, DE
• Niels Wedemeyer · Berlin, DE
• Elmar Maier · Feldkirch, AT
• Sebastian Meier-Ewert · Martinsried, DE

Key dates

• Filing date: May 30, 2000
• Grant date: Jun 14, 2005
• Expiry date: Apr 21, 2021

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5008
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to an improved method for the identification and optionally the characterisation of interacting molecules designed to detect positive clones from the rather large numbers of false positive clones isolated by conventional two-hybrid systems. The method of the invention relies on a novel combination of selection steps used to detect clones that express interacting molecules from false positive clones. The present invention provides for high-throughput interaction screens for the reliable identification of interacting molecules, which in turn can lead to the identification of substances inhibiting said interactions. Such inhibitors can find their use in the formulation of a pharmaceutical composition. The present invention further relates to kits useful for carrying out the method of the invention.