Enzymatic substrates for detecting Pseudomonas aeruginas
US6905841B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 6, 2000 |
| Grant date | Jun 14, 2005 |
| Priority date | — |
| Expiry date | Aug 2, 2021 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2337/30
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
An enzyme substrate which includes a target portion and a marker portion, hydrolysis of the substrate leading to separation of the target portion from the marker portion, and the target portion being specific for the enzyme activity being assayed. A formulation containing at least one such substrate, and a method for detecting the bacterial species Pseudomonas aeruginosa is also disclosed. The target portion of the substrate is specific for β-alanine aminopeptidase activity and the marker portion is a compound which reveals whether a hydrolysis reaction has taken place or not. The invention is particularly applicable in the field of diagnosis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.