Process for the separation of glycosylated and nonglycosylated proteins

US6906183B2 · kind B2 · utility

Assignee

• Delta Biotechnology Limited · GB

Inventors

• Jürgen Römisch · Marburg, DE
• Jörg Weisse · Allendorf (Lumda), DE
• Harald Stauss · Steffenberg, DE
• Annette Feussner · Marburg, DE

Key dates

• Filing date: Dec 14, 1999
• Grant date: Jun 14, 2005
• Expiry date: Jul 31, 2020

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/76
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process for the partial or complete separation of glycosylated and nonglycosylated proteins is described, in which: a) a triazine dye immobilized on a matrix is incubated with a mixture of glycosylated and nonglycosylated proteins, b) the matrix is then washed to remove the unbound proteins, and c) the proteins are eluted by means of a stepwise or continuous increase in the ionic strength or in the pH, nonglycosylated proteins and proteins having an increasing degree of glycosylation being collected separately from one another in the eluate fractions obtained. By means of the use of this process it is possible, for example, to prepare a human albumin which is free of glycoside bonds and is expressed in yeast cells.